Exome sequencing -- cheaper and faster, greater sequencing depth alternative to whole genome sequencing

http://en.wikipedia.org/wiki/Exome_sequencing

Hybrid capture

In-Solution Capture.

This technique involves hybridizing shotgun libraries of genomic DNA to target-specific sequences on a microarray.^[4] Roche NimbleGen was first to take this technology and adapt it for next-generation sequencing. They developed the Sequence Capture Human Exome 2.1M Array to capture ~180,000 coding exons.^[3] This method is both time-saving and cost-effective compared to PCR based methods. The Agilent Capture Array and the comparative genomic hybridization array also other methods that can be used for hybrid capture of target sequences. Limitations in this technique include the need for expensive hardware as well as a relatively large amount of DNA.^[4]

In-solution capture

To capture genomic regions of interest using in-solution capture, a pool of custom oligonucleotides (probes) is synthesized and hybridized in solution to a fragmented genomic DNA sample. The probes (labeled with beads) selectively hybridize to the genomic regions of interest after which the beads (now including the DNA fragments of interest) can be pulled down and washed to clear excess material. The beads are then removed and the genomic fragments can be sequenced allowing for selective DNA sequencing of genomic regions (e.g. exons) of interest.