Flow cytometry - Day 1

Sept 7
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FCS - forward scatter
- increasing in size of cell == increasing FSC
- increasing in refractive index of cell == increasing FSC
- bright == large refractive index

SSC - side scatter
- increasing granularity (contents in cell) == increasing SSC

Cell types:
platelets (smallest) - erythrocytes (RBC) - lymphocytes (WBC, T cells, B cells, Natural killer cells, neutrophil)

CD3 and CD4 T cell receptors

FCS - Flow Cytometry Standard

Parameters:
- FSC
- SSC
- FITC - fluorescein isothicyanate fluorochrome
- PE fluorescence - phycoerythin fluorochrome

Gating:
- restricts analysis to one population eg. snap-to-gate, quadrant, polygon

Event:
- cell passing through a laser

Compensation correction for multicolor experiments

http://www.bdbiosciences.com/support/training/itf_launch.jsp