Wednesday, October 9, 2013

Statistics on how many reads are found in the intron

Total RNA sequencing reveals nascent transcription and
widespread co-transcriptional splicing in the human brain
http://www.nature.com/nsmb/journal/v18/n12/pdf/nsmb.2143.pdf

- around 40% mapped to introns
- fetal brain > adult brain > liver

Ribo-Zero Gold Kit: improved RNA-seq results after
removal of cytoplasmic and mitochondrial ribosomal
RNA

http://www.nature.com/nmeth/journal/v8/n11/pdf/nmeth.f.352.pdf%3FWT.ec_id%3DNMETH-201111

- around 32% mapped to introns

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