Next-gen sequencing
http://seqanswers.com/forums/attachment.php?attachmentid=365&d=1274818435
http://www.nature.com.proxy.lib.sfu.ca/nrg/journal/v11/n1/abs/nrg2626.html
http://www.nature.com.proxy.lib.sfu.ca/nature/journal/v452/n7189/abs/nature06884.html
http://hmg.oxfordjournals.org.proxy.lib.sfu.ca/content/19/R2/R227.full (Third gen sequencing)
http://www.biostat.jhsph.edu/~hji/courses/genomics/Sequencing.ppt Excellent summary of Next-gen
emulsion PCR
- ABI Solid and 454 Pyro
- only one primer is used in a microbead
bridge PCR / cluster PCR / immobilized PCR
- Solexa / Illumina
- both primers / adpaters attached on immobilized flow cell surface are used
Long-Range PCR - has proofreading
http://www.springerprotocols.com.proxy.lib.sfu.ca/Abstract/doi/10.1385/1-59259-273-2:051
Ligation-mediated PCR / Linker-mediated PCR
http://nar.oxfordjournals.org.proxy.lib.sfu.ca/content/24/8/1547.full
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