A draft map of the human proteome

Nature

509,

575–581

(29 May 2014)

doi:10.1038/nature13302

Received: 09 August 2013
Accepted: 31 March 2014
Published online: 28 May 2014

Abstract

The availability of human genome sequence has transformed biomedical research over the past decade. However, an equivalent map for the human proteome with direct measurements of proteins and peptides does not exist yet. Here we present a draft map of the human proteome using high-resolution Fourier-transform mass spectrometry. In-depth proteomic profiling of 30 histologically normal human samples, including 17 adult tissues, 7 fetal tissues and 6 purified primary haematopoietic cells, resulted in identification of proteins encoded by 17,294 genes accounting for approximately 84% of the total annotated protein-coding genes in humans. A unique and comprehensive strategy for proteogenomic analysis enabled us to discover a number of novel protein-coding regions, which includes translated pseudogenes, non-coding RNAs and upstream open reading frames. This large human proteome catalogue (available as an interactive web-based resource at http://www.humanproteomemap.org) will complement available human genome and transcriptome data to accelerate biomedical research in health and disease.

Subject terms:

Tuesday, May 6, 2014

SSH Tunnelling with Putty (Windows) and Foxy Proxy

Follow these steps to allow you to view internal web pages.

Open Putty and set:

host name: myhostname.com
host port: 22
Connection > SSH > Tunnels:
- Pick a source port, say 9870
- Click Dynamic
- Click Add
Click OK to connect. Leave this session open.

Install Foxy Proxy in your web browser and set:

host or IP address: 127.0.0.1
port: 9870
Select SOCKS Proxy
Select SOCKS v5
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Point your browser to a valid URL

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Friday, May 30, 2014

Tissue specific gene expression

Wednesday, May 28, 2014